Automated roller bottle processing system is a self-contained, automated roller bottle processing system. It is suitable for all current roller bottle applications in research laboratories and large scale manufacturing sites, including the production of human and animal vaccines, clean cells for fermenter culture, and recombinant proteins for therapeutic applications. The unit has been designed to automate all stages of roller bottle based tissue culture, from cell inoculation, incubation, media change and trypsinisation to final harvest.